Progress on the Extraction, Separation, Biological Activity, and Delivery of Natural Plant Pigments.

Natural plant pigments are safe and have low toxicity, with various nutrients and biological activities. However, the extraction, preservation, and application of pigments are limited due to the instability of natural pigments. Therefore, it is necessary to examine the extraction and application processes of natural plant pigments in detail. This review discusses the classification, extraction methods, biological activities, and modification methods that could improve the stability of various pigments from plants, providing a reference for applying natural plant pigments in the industry and the cosmetics, food, and pharmaceutical industries.

Effects of coal spoil amendment on heavy metal accumulation and physiological aspects of ryegrass (Lolium perenne L.) growing in copper mine tailings.

Copper mine tailings pose many threats to the surrounding environment and human health, and thus, their remediation is fundamental. Coal spoil is the waste by-product of coal mining and characterized by low levels of metals, high content of organic matter, and many essential microelements. This study was designed to evaluate the role of coal spoil on heavy uptake and physiological responses of Lolium perenne L. grown in copper mine tailings amended with coal spoil at rates of 0, 0.5, 1, 5, 10, and 20%. The results showed that applying coal spoil to copper mine tailings decreased the diethylenetriaminepentaacetic acid (DTPA)-extractable Cd, Cu, Pb, and Zn contents in tailings and reduced those metal contents in both roots and shoots of the plant. However, application of coal spoil increased the DTPA-extractable Cr concentration in tailings and also increased Cr uptake and accumulation by Lolium perenne L. The statistical analysis of physiological parameters indicated that chlorophyll and carotenoid increased at the lower amendments of coal spoil followed by a decrease compared to their respective controls. Protein content was enhanced at all the coal spoil amendments. When treated with coal spoil, the activities of superoxide dismutases (SOD), peroxidase (POD), and catalase (CAT) responded differently. CAT activity was inhibited, but POD activity was increased with increasing amendment ratio of coal spoil. SOD activity increased up to 1% coal spoil followed by a decrease. Overall, the addition of coal spoil decreased the oxidative stress in Lolium perenne L., reflected by the reduction in malondialdehyde (MDA) contents in the plant. It is concluded that coal spoil has the potential to stabilize most metals studied in copper mine tailings and ameliorate the harmful effects in Lolium perenne L. through changing the physiological attributes of the plant grown in copper mine tailings.

Lanthanum resulted in unbalance of nutrient elements and disturbance of cell proliferation cycles in V. faba L. seedlings.

Effects of lanthanum (La) on mineral nutrients, cell cycles, and root lengthening have been little reported. The present work investigated these physiological responses in roots of Vicia faba seedlings cultivated in La3+-contained solutions for 15 days. The results showed that the increasing contents of La in the roots and leaves contributed to disbalances of contents of Ca, Fe, Cu, Zn, Mg, Mn, P, and K elements, and potential redistributions of some elements in the roots and leaves. These disbalances might be involved in the subsequent alteration of cell cycle phases in the root tips. Low-dose promotion and high-dose inhibition (Hormetic effects) were demonstrated as the dose responses of G0/G1-, S- or G2/M-phase ratios. The cell cycles were most probably arrested at G1/S interphase by La3+ in the root tips. The fact that the root lengths were not consistent with the changes of cell cycle phases suggested that the cell proliferation activities might be masked by other factors (e.g., cell expansion) under long-time exposure to La3+.

Hormesis effects and implicative application in assessment of lead-contaminated soils in roots of Vicia faba seedlings.

Chemical analyses and biological methods were combined to investigate oxidative stress, hormesis effect and concerned mechanism in roots of Viciafaba seedlings grown in 0-2000 mg kg(-1) of Pb-treated soils after germination of 20d. The results showed that U-shaped dose response curves were displayed in superoxide radical (O2-) radicals, guaiacol peroxidase (POD) and ascorbate peroxidase (APX) activities, malondialdehyde (MDA) and carbonyl groups as well as activities of endoproteinase (EP) isoenzymes in the roots at low doses of extraneous Pb, indicating reduced oxidative stress and toxic effect. The inverted U-shaped curves were also exhibited in growth height, superoxide dismutase (SOD) and EP activities as well as inducible heat shock protein70 (HSP70) with the increasing extraneous Pb, indicative of enhanced oxidative stress. The enhancement in HSP70, carbonyl groups and EP activities confirmed intracellular proteotoxicity and proteolytic activity in the roots at higher doses of soil Pb. More interestingly, levels of inducible HSP70 were well correlated with those of growth heights (r=0.809, p<0.05), implying that HSP70 induction may be one of the mechanisms underlying the U-shaped growth curve of V. faba seedlings in the experiment. The results suggest that traditional threshold models ought to be combined with hormesis effect in assessment of Pb-polluted soils and the threshold dose range of Pb-treated soils is proposed rudimentally as 25-125 mg kg(-1).

[Allelopathy and chemical constituents of Ligularia virgaurea volatile].

Ligularia virgaurea is a noxious weed widely distributed on the psychro-grasslands of east Qinghai-Tibet Plateau of China, but the allelopathic effects of its volatile is less known. In this study, the allelopathy of L. virgaurea volatile to 5 kinds of grasses was examined, and its chemical constituents were analyzed by GC-MS. The results demonstrated that the volatile of growing L. virgaurea could inhibit the seed germination speed and germination rate of all tested grasses. The inhibitory effects on Elymus nutans and Bromus magnus were significant, while Poa annua and Festuca ovin were not very sensitive. 18 major constituents were identified from the essential oil of L. virgaurea, accounted for 68.24% of the total. The main chemical components were 2-methylheptane (9.84%), 3-methyl-heptane (8.25%), heptane (7.93%), 4-methyl-1-(methylethyl)-bicyclo [3, 1, 0] hex-2-ene (7.79%), 3-methyl-hexane (6.38%), 2-methyl-hexane (5.54%), and D-limonene (4.70%). Monoterpenoids accounted for 16.58% of the total, indicating that volatilization was one of the ways by which L. virgaurea released allelochemicals. The allelopathy of the volatile may play an important role in enhancing the competitive ability of the species, and be one of reasons of grassland degeneration.